Jump to ContentJump to Main Navigation
Meselson, Stahl, and the Replication of DNAA History of "The Most Beautiful Experiment in Biology"$
Users without a subscription are not able to see the full content.

Frederic Lawrence Holmes

Print publication date: 2001

Print ISBN-13: 9780300085402

Published to Yale Scholarship Online: October 2013

DOI: 10.12987/yale/9780300085402.001.0001

Show Summary Details
Page of

PRINTED FROM YALE SCHOLARSHIP ONLINE (www.yale.universitypressscholarship.com). (c) Copyright Yale University Press, 2021. All Rights Reserved. An individual user may print out a PDF of a single chapter of a monograph in YSO for personal use.date: 25 September 2021

One Discovery, Three Stories

One Discovery, Three Stories

Chapter:
(p.303) Chapter Nine One Discovery, Three Stories
Source:
Meselson, Stahl, and the Replication of DNA
Author(s):

Frederic Lawrence Holmes

Publisher:
Yale University Press
DOI:10.12987/yale/9780300085402.003.0010

This chapter discusses Meselson's and Stahl's decision to switch from 5-BU to 15N to produce heavy DNA and, at the same time, to carry out the transfer experiments with bacteria in place of phage. Their shift, although abrupt, was premeditated. Meselson had already contemplated using bacteria earlier when he still envisioned carrying out the experiments only with 5-BU. In the research statement he submitted, he outlined the strategy he would use. The plan was to grow the organisms in the heavy medium, transfer them to the light medium, withdraw samples at time intervals representing successive cell generations, lyse the bacteria to release their DNA, and centrifuge the latter in the density gradient. The first step in the new experimental design was to test whether Escherichia coli would grow normally in a medium containing heavy nitrogen.

Keywords:   5-BU, 15N, heavy DNA, transfer experiments, bacteria, phage, successive cell generations, density gradient, Escherichia coli

Yale Scholarship Online requires a subscription or purchase to access the full text of books within the service. Public users can however freely search the site and view the abstracts and keywords for each book and chapter.

Please, subscribe or login to access full text content.

If you think you should have access to this title, please contact your librarian.

To troubleshoot, please check our FAQs , and if you can't find the answer there, please contact us.